Labeled Probes

RADIOLABELED and Non-Radiolabeled PROBES-DNA


	Radiolabeled & Non-radiolabeled Probes Our Radiolabeling Department can transcribe any plasmid templates or PCR amplified DNA fragments by either Nick Translation, Random Priming, Klenow fill-in, T4-PNK or Terminal Transferase and label the RNA trasnscripts with ³²P, ³³P, ³⁵S, biotin, or digoxigenin (DIG). Methods have been developed and validated in our laboratory for the production of probes for use in In Situ Hybridization experiments.
Catalog No.	Custom DNA Labeling
L3-102	³²P-Standard Reaction (up to 100 ng of DNA )
L3-102D	³²P-Double Reaction (up to 200 ng of DNA )
L3-102K	³²P-Klenow Fill-In Reaction
L3-102LM	DNA Isolation-Low Melt Gel
L3-101	Nick Translation ³²P-Standard Reaction (up to 1000 ng of DNA)
L3-101D	Nick Translation ³²P-Double Reaction (up to 2000 ng of DNA)
L3-1021	End Labeling ³²P-T4-Polynucleotide Kinase Reaction
L3-1023	³²P-Terminal Transferase Reaction
Other isotopes are available. Please Inquire

NON-RADIOLABELED PROBES-DNA
Catalog No.	Custom DNA End Labeling
L3-1023B	Biotin-dUTP Terminal Transferase Reaction
L3-1023D	Digoxigenin-dUTP Terminal Transferase Reaction
Catalog No.	Custom DNA Random Prime Labeling
L3-102BIO	Biotin-dUTP RANDOM PRIME Reaction
L3-102DIG	Digoxigenin-dUTP RANDOM PRIME Reaction
Other lables are available. Please Inquire View/Submit Probes Data Card

DNA PROBE LABELING - DATA CARD

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For Radom Primer Extension:
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For Nick Translation:
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For End Labelings: (Up to 40 pmol per reaction)	Number of bases: Is 5' phosphate present?
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	To Print a Copy of This Data Card Before Submiting, Highlight the Area to be Printed and Choose "Print Selection" from Your Printer Dialog Box.
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DNA ISOLATION, RNA ISOLATION, PLASMID DNA ISOLATION, RECOMBINANT LIBRARY, SOUTHERN BLOTS, NORTHERN BLOTS, WESTERN BLOTS